Carboxy Flexi System, Transfer

by Promega
Available SKUs: C9320 |
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Description

  • Overview
  • Protocols
  • Specifications
  • Resources
  • The Flexi® Vector System is a directional cloning method for protein-coding sequences. It is based on two rare-cutting restriction enzymes, SgfI and PmeI, and provides a rapid, efficient and high-fidelity way to transfer protein-coding regions without the need to resequence. Once an ORF is cloned, conveniently transfer the inserts into a variety of vectors designed for multiple applications. No licensing fees or complicated transfer restrictions needed.

    All Flexi® Vectors carry the lethal barnase gene, which is replaced by the DNA fragment of interest and acts as a positive selection for the successful ligation of the insert.

    Unlike site-specific recombination vector systems, the Flexi® Vector Systems do not require appending multiple amino acids to the amino or carboxy termini of the protein of interest. In addition, the systems do not require an archival entry vector, and most applications allow direct entry into the vector suited to the experimental design.

    C-terminal Flexi® Vectors allow expression of C-terminal-tagged proteins. While these vectors can act as acceptors of protein-coding regions flanked by SgfI and PmeI, they lack a PmeI site and contain a different blunt-ended site, EcoICRI. This joined sequence cannot be removed from the C-terminal Flexi® Vectors and transferred to other Flexi® Vectors.

    Cloning a protein-coding region into the Flexi® Vectors.
    Cloning a protein-coding region into the Flexi® Vectors.
    Transfer of a protein-coding region between N-terminal or native Flexi® Vectors.
    Transfer of a protein-coding region between N-terminal or native Flexi® Vectors.

    For more information about the HaloTag® Flexi® Vectors, please visit the HaloTag® Technology page.

    Note: For access to 20,000 human and 15,000 mouse ORF clones with HaloTag® Technology in multiple mammalian expression vectors and custom cloning options, visit: www.genecopoeia.com and www.kasuza.or.jp.kop/dsearch-e

  • Protocols

    Complete Protocol

    Download PDF

    Flexi® Vector Systems Technical Manual

    PDF (859 KB)

    Video Protocols

    flexi01-fallback
  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    COMPONENTS AT DIFFERENT TEMPS

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

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