CaspACE Assay System, Colorimetric

by Promega
Available SKUs: G7220 | G7351 |
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Description

  • Overview
  • Protocols
  • Specifications
  • Resources
  • The CaspACE™ Assay System, Colorimetric, provides reagents for measuring the activity of caspase-3. The system includes a colorimetric substrate and a cell-permeant inhibitor that allow quantitative measurement of caspase-3 (DEVDase) protease activity. The colorimetric substrate (Ac-DEVD-pNA) provided is labeled with the chromophore p-nitroaniline (pNA). pNA is released from the substrate upon cleavage by DEVDase. Free pNA produces a yellow color that is monitored by a spectrophotometer at 405nm. The amount of yellow color produced upon cleavage is proportional to the amount of DEVDase activity present in the sample.

    The potent, irreversible and cell-permeant pan-caspase inhibitor Z-VAD-FMK is provided in the CaspACE™ Assay System, Colorimetric. The addition of the Z-VAD-FMK Inhibitor prior to the induction of apoptosis in cell culture inhibits the activation of the caspase cascade, including caspase-3.

  • Protocols

    Complete Protocol

    Download PDF

    CaspACE™ Assay System, Colorimetric Technical Bulletin

    PDF (522 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

    Citations

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