- Overview
- Protocols
- Specifications
- Resources
How Does Caspase-Glo® 1 Work?
The Caspase-Glo® 1 Inflammasome Assay is a simple, homogeneous, bioluminescent method to selectively measure the activity of caspase-1, a member of the cysteine aspartic acid-specific protease (caspase) family and an essential component of the inflammasome.
Inflammasomes are protein complexes induced by diverse inflammatory stimuli. Innate immune cells respond to pathogens and other danger signals with inflammasome formation and conversion of procaspase-1 zymogen into catalytically active caspase-1. Caspase-1 activation results in the processing and release of cytokines IL-1? and IL-18, as well as pyroptosis, an immunogenic form of cell death. The Caspase-Glo 1 assay is sensitive enough to measure caspase-1 activity directly in cells or in medium in multiwell plates.
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Protocols
Complete Protocol
Caspase-Glo® 1 Inflammasome Assay
PDF (2 MB)
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Certificate of Analysis
Lookup Certificate of AnalysisStorage Conditions
-30C TO -10C
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
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Resources
Articles
- Easier Detection of Inflammasome Activation
- Measuring Luminescence Using the Caspase-Glo® 1 Inflammasome Assay with the GloMax® Discover System
Citations
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A bioluminescent caspase-1 activity assay rapidly monitors inflammasome activation in cells.
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2017 J. Immunol. Methods
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