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Caspase-Glo 6 Assay

by Promega
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  • Overview
  • Protocols
  • Specifications
  • Resources
  • Caspase-6 is a member of the cysteine aspartic acid-specific protease family and has a key effector role in the cleavage of specific target proteins during apoptosis. The Caspase-Glo® 6 Assay provides a luminogenic substrate, Z-VEID-aminoluciferin, in a buffer optimized for caspase-6 and luciferase activity. The addition of a single Caspase-Glo® 6 Reagent in an add-mix-measure format results in cleavage of the substrate, releasing aminoluciferin, and generation of a glow-type luminescent signal in the presence of Ultra-Glo™ Recombinant Luciferase. The luminescent signal is proportional to the amount of caspase-6 activity present. The homogeneous Caspase-Glo® 6 Assay is designed for use with purified enzyme preparations in multiwell plate formats, making it ideal for automated high-throughput screening for caspase-6 activity and inhibitors of caspase-6 activity.

    Features and benefits

    •  Increased accuracy and superior sensitivity over fluorescence-based caspase assays allows inhibitor studies at concentrations below the Km.
    • The maximum signal (and maximum sensitivity) of the assay is reached in as little as 30 minutes after reagent addition.
    • The assay demonstrates an excellent Z´-factor value of 0.86 when using 0.1U/ml of caspase-6 for assays in 384-well plates.
    • The coupled-enzyme, homogeneous format results in a continuous signal, providing excellent stability and allowing plates to be read over an extended period of time.
    Caspase-6 cleavage of the luminogenic substrate containing the VEID consensus sequence.
    Caspase-6 cleavage of the luminogenic substrate containing the VEID consensus sequence.

  • Protocols

    Complete Protocol

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    Caspase-Glo® 6 Assay Technical Bulletin

    PDF (983 KB)

  • Certificate of Analysis

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    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources