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cGMP-Dependent Protein Kinase (alpha-Isozyme)

by Promega
€285
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  • Overview
  • Protocols
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  • cGMP-Dependent Protein Kinase is a serine/threonine protein kinase present in smooth muscle and a variety of other tissues. The kinase is a 78kDa polypeptide composed of a regulatory domain and a catalytic domain and is active as a homodimer.

    Use cGMP-Dependent Protein Kinase to investigate the role of target protein phosphorylation in cell regulation and signal transduction. Applications include microinjection studies of the role of cGMP-Dependent Protein Kinase in cellular processes;  in vitro enzymological studies of protein phosphorylation; and studies on cGMP-Dependent Protein Kinase substrates and inhibitors. 

    Storage Buffer: 10mM potassium phosphate (pH 6.8), 1mM EDTA, 25mM ?-mercaptoethanol, 0.15M NaCl, 12% sucrose.

    Unit Definition: One unit is defined as the amount of cGMP-Dependent Protein Kinase required to catalyze incorporation of 1pmol of phosphate into a heptapeptide substrate (RKRSRAE) in one minute at 30°C.

    Properties:

    • Specific Activity: >1,000u/ug (kinase activity).
    • Highly Pure: cGMP-Dependent Protein Kinase has been purified by the method of Corbin and Doskeland and is >90% pure as determined by SDS-PAGE (single band).

    Reference:

    1. Corbin, J.D. and Doskeland, S.O. (1983) J. Biol. Chem. 258, 11391–7.
  • Protocols

    Complete Protocol

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    cGMP-Dependent Protein Kinase (Alpha Isozyme) Product Information

    PDF (115 KB)

  • Certificate of Analysis

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    Storage Conditions

    LESS THAN -65C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

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