Chymotrypsin, Sequencing Grade

by Promega
Available SKUs: V1061 | V1062 |
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Description

  • Overview
  • Protocols
  • Specifications
  • Resources
  • A highly-purified serine endopeptidase derived from bovine pancreas that preferentially cleaves at the carboxyl side of aromatic amino acids Tyr, Phe and Trp. Cleavage may also be observed, but at a lower rate, at Leu and Met. Chymotrypsin activity is optimal at pH of 7.0–9.0. This sequencing grade enzyme can be used alone or in combination with other proteases to produce protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is suitable for digestion reactions in-solution or in-gel.

    Applications
    • Peptide mapping
    • Protein identification
    Cut sites for Arg-C, Sequencing Grade; Glu-C, Sequencing Grade; Asp-N, Sequencing Grade; Trypsin Gold, Mass Spectrometry Grade; Sequencing Grade Modified Trypsin; Immobilized Trypsin; Chymotrypsin, Sequencing Grade; Endoproteinase Lys-C, Sequencing Grade; rLys-C, Mass Spec Grade.
    Cut sites for Arg-C, Sequencing Grade; Glu-C, Sequencing Grade; Asp-N, Sequencing Grade; Trypsin Gold, Mass Spectrometry Grade; Sequencing Grade Modified Trypsin; Immobilized Trypsin; Chymotrypsin, Sequencing Grade; Endoproteinase Lys-C, Sequencing Grade; rLys-C, Mass Spec Grade.
  • Protocols

    Complete Protocol

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    Chymotrypsin Sequencing Grade Product Information

    PDF (123 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    +2C TO +10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

    Articles

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