Description
- Overview
- Protocols
- Specifications
- Resources
Convenient, Scalable Kinase Profiling
The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The CK1? Kinase Enzyme System contains:
- CK1? Kinase, 10ug (Human, recombinant full-length). MW: ~72kDa.
- Casein, dephosphorylated, Substrate; native protein purified from bovine milk.
- Reaction Buffer, DTT.
Full-length recombinant human CK1? (CK1epsilon) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CK1? is a member of the CK1 family of serine/threonine protein kinases, which play an important role in diverse cell processes, including DNA replication and repair. CK1? is a regulator of Yes-associated protein (YAP) transcription coactivator, which is a key regulator of organ size and a candidate human oncogene. CK1? is activated by CCK2R, and this then phosphorylates PKD2 at Ser244. Phosphorylation of PKD2 leads to its nuclear accumulation and efficient phosphorylation of nuclear PKD2 substrates in human gastric cancer cells. CKI? can phosphorylate topoisomerase (topo) IIalpha at serine-1106, and this regulates the enzyme activity and sensitivity to topo II-targeted drugs.
The CK1? Kinase Enzyme System can be purchased with or without the ADP-Glo™ Kinase Assay reagents. Used together, the ADP-Glo™ Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.
Use with ADP-Glo™ Kinase Assay
The ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.
See all Kinase Enzyme Systems available from Promega.
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Protocols
Complete Protocol
CK1 epsilon Kinase Enzyme Protocol
PDF (917 KB)
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Certificate of Analysis
Lookup Certificate of AnalysisStorage Conditions
LESS THAN -65C
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
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Resources
Posters
- Bringing Assays and Automation Together for Lower Throughput Kinase Profiling Poster
- Automation of ADP-Glo Universal Kinase Assay For HTS Screening AND Profiling Applications Poster
Other
