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CytoTox-Fluor Cytotoxicity Assay

by Promega
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  • Overview
  • Protocols
  • Specifications
  • Resources
  • The CytoTox-Fluor™ Cytotoxicity Assay is a single-reagent-addition, homogeneous, fluorescent assay that measures the relative number of dead cells in cell populations. The assay measures a distinct protease activity associated with cytotoxicity and uses a fluorogenic peptide substrate (bis-alanyl-alanyl-phenylanlanyl-rhodamine 110; bis-AAF-R110) to measure "dead-cell activity," which has been released from cells that have lost membrane integrity. The bis-AAF-R110 substrate cannot cross the intact membrane of live cells and therefore gives no signal from live cells. The assay is designed to accommodate downstream multiplexing with several Promega luminescent assays or spectrally distinct fluorescent assay methods, such as assays to measure caspase activation, reporter gene expression or orthogonal measures of viability.

    Features and Benefits

    • Homogeneous, "add-mix-measure" protocol eliminates parallel plate processing and reduces cell culture costs.
    • Multiplex the CytoTox-Fluor™ Assay with several Promega luminescent cell-based assays and get more data from every well.
    • Data normalization for dead-cell number makes results more comparable well-to-well, plate-to-plate, day-to-day.
    • Reduce assay variability and avoid the cumulative error associated with multistep protocols.

    G9260 contains sufficient reagents for 100 assays at 100ul/assay in a 96-well format or 400 assays at 25ul/assay in a 384-well format. G9261 contains sufficient reagents for 500 assays at 100ul/assay in a 96-well format or 2,000 assays at 25ul/assay in 384-well format. G9262 contains sufficient reagents for 1,000 assays at 100ul/assay in a 96-well format or 4,000 assays at 25ul/assay in a 384-well format.

  • Protocols

    Complete Protocol

    Download PDF

    CytoTox-Fluor™ Cytotoxicity Assay

    PDF (610 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources