6 x 5 LC-MS/MS Peptide Reference Mix
by Promega
Available SKUs:
V7491
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V7495
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Description
- Overview
- Protocols
- Specifications
- Resources
A Complete Solution for Monitoring LC and MS Instrument Quality Control and Performance
In combination with an accompanying software tool, the 6 × 5 LC-MS/MS Peptide Reference Mix provides a convenient way to assess LC column performance and MS instrument parameters, including sensitivity and dynamic range.
- Consists of 30 peptides; 6 sets of 5 isotopologues of the same peptide sequence, differing only in the number of stable, heavy-labeled amino acids incorporated into the sequence
- Labels are uniform 13C and 15N atoms.
- Isotopologues are chromatographically indistinguishable; however, since they differ in mass, they are clearly resolved by mass spectrometry.
- The isotopologues of each peptide are present in a series of tenfold dilutions. This format allows assessment of instrument dynamic range and sensitivity from a single run.
The 6 × 5 LC-MS/MS Peptides are AAA-qualified for accurate monitoring, and are compatible with multiple applications and neat or complex mixtures.
Read an ACS Analytical Chemistry article by J. Beri et al. featuring use of the 6 x 5 Reagent: Reagent for Evaluating Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) Performance in Bottom-Up Proteomic Experiments.
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Protocols
Complete Protocol
6 x 5 LC-MS/MS Peptide Reference Mix
PDF (4 MB)
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Certificate of Analysis
Lookup Certificate of AnalysisStorage Conditions
-30C TO -10C
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
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Resources
Articles
Software and Firmware
Posters
- A Novel 6 x 5 Peptide Mix for LC-MS-MS Performance Monitoring Poster
- A Novel 6 x 5 Peptide Mixture for Instrument Performance Monitoring Poster
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