DdeI

Cut Site:

C^?TNA  G
G  ANT_?C

Notes:

1. For site-specific methylation information, consult McClelland, M., Nelson, M. and Raschke, E. (1994) Nucl. Acids Res. 22, 3640–59.
2. DdeI produces an ambiguous extension, which is difficult to ligate.

Incubation Conditions: Buffer D. 37°C.

Source: Desulfovibrio desulfuricans.

Storage Buffer: 10mM Tris-HCl (pH 7.4), 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 0.01% Triton® X-100 and 50% glycerol.

Percent Activity in 4-CORE® Buffer:

Frequency of Cutting: