- Overview
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Cut Site:
C?TNA G
G ANT?CNotes:
- For site-specific methylation information, consult McClelland, M., Nelson, M. and Raschke, E. (1994) Nucl. Acids Res. 22, 3640–59.
- DdeI produces an ambiguous extension, which is difficult to ligate.
Incubation Conditions: Buffer D. 37°C.
Source: Desulfovibrio desulfuricans.
Storage Buffer: 10mM Tris-HCl (pH 7.4), 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 0.01% Triton® X-100 and 50% glycerol.
Percent Activity in 4-CORE® Buffer:
A B C D MULTI-CORE™ 25–50% 25–50% 50–75% 100% 25–50% Frequency of Cutting:
? Ad-2 ?X174 pUC18 M13mp18 pBR322 104 97 14 6 29 8 -
Protocols
Complete Protocol
Restriction Enzyme Protocol
PDF (410 KB)
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Certificate of Analysis
Lookup Certificate of AnalysisStorage Conditions
-30C TO -10C
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
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Resources
Other