DdeI

Description

• Overview
• Protocols
• Specifications
• Resources

• Cut Site:

  C^?TNA  G
  G  ANT_?C

  Notes:

  1. For site-specific methylation information, consult McClelland, M., Nelson, M. and Raschke, E. (1994) Nucl. Acids Res. 22, 3640–59.
  2. DdeI produces an ambiguous extension, which is difficult to ligate.

  Incubation Conditions: Buffer D. 37°C.

  Source: Desulfovibrio desulfuricans.

  Storage Buffer: 10mM Tris-HCl (pH 7.4), 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 0.01% Triton® X-100 and 50% glycerol.

  Percent Activity in 4-CORE® Buffer:

  A	B	C	D	MULTI-CORE™
  25–50%	25–50%	50–75%	100%	25–50%

  Frequency of Cutting:

  ?	Ad-2	?X174	pUC18	M13mp18	pBR322
  104	97	14	6	29	8

• Protocols

  Complete Protocol

  

  Restriction Enzyme Protocol

  PDF (410 KB)

• Certificate of Analysis

  Lookup Certificate of Analysis

  Storage Conditions

  -30C TO -10C

  Use Restrictions

  For Research Use Only. Not for Use in Diagnostic Procedures.

• Resources

  

  Other

  • Restriction Enzyme Resource