DeadEnd Fluorometric TUNEL System

Description

• Overview
• Protocols
• Specifications
• Resources

• The DeadEnd™ Fluorometric TUNEL System is a classic TUNEL Assay designed for the specific detection and quantitation of apoptotic cells within a cell population. This system measures nuclear DNA fragmentation, an important biochemical hallmark of apoptosis in many cell types, providing simple, accurate and rapid detection of apoptotic cells in situ at the single-cell level or in cell suspensions. The DeadEnd™ Fluorometric TUNEL System measures the fragmented DNA of apoptotic cells by catalytically incorporating fluorescein-12-dUTP at 3´-OH DNA ends using Terminal Deoxynucleotidyl Transferase (TdT), which forms a polymeric tail using the principle of the TUNEL (TdT-mediated dUTP Nick-End Labeling) assay. The fluorescein-12-dUTP-labeled DNA can then be visualized directly by fluorescence microscopy or quantitated by flow cytometry.

   Features and Benefits

  • System provides sufficient reagents for 60 assays of 50ul each and saves money.
  • Direct incorporation of fluorescent nucleotide reduces number of incubation steps.
  • Use to detect apoptosis in cultured cells and formalin-fixed, paraffin-embedded tissue sections.
  • Plastic coverslips provided simplify sample handling.

  Note: The protocol for the DeadEnd™ TUNEL Assay recommends an optional DNase I treatment of samples as a positive control to detect DNA fragmentation. RQ1 RNase-Free DNase (Cat.# M6101) can be used to generate the positive control and is available separately.

  For more information, see the Apoptosis Section of the Protocols & Applications Guide.

• Protocols

  Complete Protocol

  

  DeadEnd™ Fluorometric TUNEL System Technical Bulletin

  PDF (380 KB)

  Quick Protocols

  

  DeadEnd Fluorometric TUNEL System Quick Protocol FB055

  PDF (72 KB)

• Certificate of Analysis

  Lookup Certificate of Analysis

  Storage Conditions

  -30C TO -10C

  Use Restrictions

  For Research Use Only. Not for Use in Diagnostic Procedures.

• Resources

  

  Articles

  • Using Terminal Deoxynucleotidyl Transferase TdT Enzyme to Detect TUNEL-Positive, GFP-Expressing Apoptotic Cells
  • An Alternative, Non-Apoptotic Form of Programmed Cell Death
  • Polycystin1 the Gene Product of PKD1 Induces Resistance to Apoptosis and Spontaneous Tubulogenesis in MDCK Cells
  

  Citations

  • A long noncoding RNA regulates photoperiod-sensitive male sterility, an essential component of hybrid rice

    
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    2012 Proc. Natl. Acad. Sci. USA