Diamond Nucleic Acid Dye

by Promega
Available SKUs: H1181 |
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Description

  • Overview
  • Protocols
  • Specifications
  • Resources
  • Diamond™ Nucleic Acid Dye

    Diamond™ Nucleic Acid Dye is a sensitive fluorescent dye that binds to single-stranded DNA, double-stranded DNA and RNA, and can be used to stain and visualize nucleic acids in gels. Diamond™ Nucleic Acid Dye is compatible with denaturing and native agarose and polyacrylamide gels and can be imaged with any standard imaging system, such as by UV transillumination with a Polaroid® or digital camera, GE ImageQuant™ or Bio-Rad Gel Doc™ systems.

    The concentrated dye is stable for up to 90 days at room temperature. Diamond™ Nucleic Acid Dye does not require prewashing or destaining of gels. It is more much more sensitive than ethidium bromide, so less sample nucleic acid and nucleic acid markers are required for visualization, resulting in increased savings with every gel you run.

    Overview of the Diamond™ Nucleic Acid Dye protocol.
    Overview of the Diamond™ Nucleic Acid Dye protocol.
    Diamond™ Nucleic Acid Dye staining of DNA separated on a 1.2% Clear E-gel®.
    Diamond™ Nucleic Acid Dye staining of DNA separated on a 1.2% Clear E-gel®.

    Lane 1: 10µl of BenchTop 1kb DNA Ladder (Cat.# G7541); lane 2–12: twofold serial dilutions of ladder in 1X Blue/Orange Loading Dye (Cat.# G1881). Following electrophoresis, the gel was incubated in 1X Diamond™ Nucleic Acid Dye for 20 minutes and imaged.

    Diamond™ Gel Nucleic Acid staining of DNA separated on a 4–20% polyacrylamide gel.
    Diamond™ Gel Nucleic Acid staining of DNA separated on a 4–20% polyacrylamide gel.

    Lane 1: 10µl of BenchTop 1kb DNA Ladder (Cat.# G7541); lane 2–10: twofold serial dilutions of ladder in 1X Blue/Orange Loading Dye (Cat.# G1881). Following electrophoresis, the gel was incubated in 1X Diamond™ Nucleic Acid Dye for 20 minutes and imaged.

  • Protocols

    Complete Protocol

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    Diamond™ Nucleic Acid Dye Technical Manual

    PDF (1 MB)

    Quick Protocols

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    Diamond Nucleic Acid Dye FB142

    PDF (97 KB)

  • Certificate of Analysis

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    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

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