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Donkey Anti-Goat IgG, HRP

by Promega
€179
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  • Overview
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  • Specifications
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  • Polyclonal secondary antibody raised in donkey, immunoaffinity-purified using immobilized antigens and conjugated to horseradish peroxidase (HRP) enzyme.

    Donkey Anti-Goat IgG, HRP Conjugate is a secondary antibody developed in donkeys against goat IgG. This antibody shows reactivity to goat and sheep IgG but minimal cross-reactivity to rabbit and mouse IgG. For Western blot applications with chromogenic detection, use the secondary antibody at a starting dilution of 1:10,000. As with all antibodies, in certain applications some species-dependent antigen-dependent cross-reactivity may be observed. The product (unless otherwise noted) is supplied as a 1mg/ml solution. The optimum concentration of secondary antibody depends on the application and will need to be empirically determined.

    We can readily accommodate large-scale custom orders. Please inquire at: www.promega.com/custom/

    Applications

    • Western blotting.
    • Enzyme-linked immunosorbent assay (ELISA).
    • Dot blotting.

    References

    1. Hu, D. et al. (2012) Novel insight into KLF4 proteolytic regulation in estrogen receptor signaling and breast carcinogenesis. J. Biol. Chem. 287, 13584–97.
    2. Hesse, E. et al. (2010) Zfp521 controls bone mass by HDAC3-dependent attenuation of Runx2 activity. J. Cell Biol. 191, 1271–83.
    3. Massaad, C.A., Washington, T.M., Pautler, R.G. and Klann, E. (2009) Overexpression of SOD-2 reduces hippocampal superoxide and prevents memory deficits in a mouse model of Alzheimer's disease. Proc. Natl. Acad. Sci. USA 106, 13576–81.
    4. Berkowitz, O., Jost, R., Pollmann, S. and Masle, J. (2008) Characterization of TCTP, the translationally controlled tumor protein, from Arabidopsis thaliana. Plant Cell 20, 3430–47.
    5. Robertson, J.B., Stowers, C.C., Boczko, E. and Johnson, C.H. (2008) Real-time luminescence monitoring of cell-cycle and respiratory oscillations in yeast. Proc. Natl. Acad. Sci. USA 105, 17988–93.
  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

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