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DpnI

by Promega
€80
Size
  • Overview
  • Protocols
  • Specifications
  • Resources
  • Cut Site:

    GmeA?TC
    CT?meAG

    Usage Note: DpnI requires N6-methylation of the adenine residue for activity, unlike its isoschizomers, MboI and Sau3AI.

    Incubation Conditions: Buffer B. 37°C.

    Source: Diplococcus pneumoniae.

    Storage Buffer: 10mM Tris-HCl (pH 7.4), 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

    Percent Activity in 4-CORE® Buffer:

    A B C D MULTI-CORE™
    50–75% 100% 75–100% 50–75% 100%

    Frequency of Cutting:

    ? Ad-2 ?X174 pUC18 M13mp18 pBR322
    116 87 0 15 7 22
  • Protocols

    Complete Protocol

    Download PDF

    Restriction Enzyme Protocol

    PDF (410 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

    Other