ECL Western Blotting Substrate

by Promega
Available SKUs: W1001 | W1015 |
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Description

  • Overview
  • Protocols
  • Specifications
  • Resources
  • Highly sensitive non-radioactive, enhanced luminol-based chemiluminescent substrate for the detection of horseradish peroxidase (HRP) conjugates on immunoblots. The ECL Western Blotting Substrate detects and visualizes the presence of picogram (pg) amounts of antigen through the use of photographic or other suitable chemiluminescent imaging methods.

    No optimization required; easy to switch from other entry-level ECL substrates.

    Applications
    • Western blotting
    • Dot blotting
    Western blot (immunoblot) for ?-actin in cytoplasmic lysate from HEK293T cells.
    Western blot (immunoblot) for ?-actin in cytoplasmic lysate from HEK293T cells.
  • Protocols

    Complete Protocol

    Download PDF

    ECL Western Blotting Substrate Technical Manual

    PDF (112 KB)

    Quick Protocols

    Download PDF

    ECL Western Blotting Substrate Quick Protocol FB102

    PDF (49 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    +2C TO +10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

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