EcoRI

by Promega
Available SKUs: R6011 |
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Description

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  • Overview
  • Protocols
  • Specifications
  • Resources
  • Cut Site:

    G?AATT   C
    C   TTAA?G

    Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/

    Note: Star activity may be observed with glycerol concentrations >12%, enzyme:DNA ratios >25u/ug, in the absence of NaCl, in the presence of Mn2+, or under high pH conditions.

    Incubation Conditions: Buffer H. 37°C.

    Source: Escherichia coli RY 13.

    Storage Buffer: 10mM Tris-HCl (pH 7.4), 400mM NaCl, 0.1mM EDTA, 1mM DTT, 0.15% Triton® X-100, 0.5mg/ml BSA, 50% glycerol.

    Percent Activity in 4-CORE® Buffer:

    A B C D H MULTI-CORE™
    25–50% 50–75% 50–75% 50–75% 100% 100%*

    *MULTI-CORE™ Buffer is not recommended due to potential star activity.

    Frequency of Cutting:

    ? Ad-2 ?X174 pUC18 M13mp18 pBR322
    5 5 0 1 1 1
  • Protocols

    Complete Protocol

    Download PDF

    Restriction Enzyme Protocol

    PDF (410 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

    Citations

    Other

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