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Note: Star activity may be observed with glycerol concentrations >12%, enzyme:DNA ratios >25u/ug, in the absence of NaCl, in the presence of Mn2+, or under high pH conditions.
Incubation Conditions: Buffer H. 37°C.
Source: Escherichia coli RY 13.
Storage Buffer: 10mM Tris-HCl (pH 7.4), 400mM NaCl, 0.1mM EDTA, 1mM DTT, 0.15% Triton® X-100, 0.5mg/ml BSA, 50% glycerol.
Percent Activity in 4-CORE® Buffer:
A B C D H MULTI-CORE™ 25–50% 50–75% 50–75% 50–75% 100% 100%*
*MULTI-CORE™ Buffer is not recommended due to potential star activity.
Frequency of Cutting:
? Ad-2 ?X174 pUC18 M13mp18 pBR322 5 5 0 1 1 1
Restriction Enzyme Protocol
PDF (410 KB)
Certificate of AnalysisLookup Certificate of Analysis
-30C TO -10C
For Research Use Only. Not for Use in Diagnostic Procedures.