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EnduRen Live Cell Substrate

by Promega
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  • Overview
  • Protocols
  • Specifications
  • Resources
  • Stable Luminescence Detection for Live-Cell, High-Throughput Assays

    EnduRen™ Live Cell Substrate enables live cell kinetic measurements, streamlining assay development and multiplexing with other lytic assays. EnduRen provides the ability to measure Renilla luciferase luminescence for at least 24 hours after substrate addition, with up to tenfold higher signal-to-background ratios than wildtype coelenterazines.

    EnduRen™ Live Cell Substrate is a uniquely engineered coelenterazine with protected oxidation sites, which minimizes substrate degradation and autoluminescence (background) in cell culture, while it extends the luminescent signal to accommodate microplates without the need for auto-injectors. The result is that EnduRen™ Substrate overcomes the key limitations of wildtype coelenterazines by providing an automation-friendly, highly sensitive substrate for Renilla luciferase-based gene reporter and BRET applications.

    Assay Advantages

    • Conserve test compounds as you create response profiles in real time to generate more data-rich results.
    • Rapidly obtain optimal assay parameters through repeat measurements using only a single cell population. Increase your sample throughput using microplates without time-consuming per-sample reagent injection steps.
    • Perform more dynamic experiments using the same sample set by pairing with any lytic assay.
    • Reliably quantitate low levels of expression for reporter gene detection and BRET.
  • Protocols

    Complete Protocol

    Download PDF

    EnduRen™ Live Cell Substrate Technical Manual

    PDF (661 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources