AccI

by Promega
Available SKUs: R6411 | R6415 |
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Description

  • Overview
  • Protocols
  • Specifications
  • Resources
  • Cut Site:

    GT?(A/C)(T/G)   AC
    CA   (T/G)(A/C)?TG

    Note: For site-specific methylation information, consult Nelson, M. and McClelland, M. (1991) Nucl. Acids Res.s19, 2045–71.

    Incubation Conditions: Buffer G. 37°C.

    Source: Acinetobacter calcoaceticus.

    Storage Buffer: 10mM Tris-HCl (pH 7.4), 50mM NaCl, 10mM MgCl2, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

    Percent Activity in 4-CORE® Buffer:

    A B C D G MULTI-CORE™
    50–75% 25–50% 25–50% 10–25% 100% 25–50%

    Frequency of Cutting:

    ? Ad-2 ?X174 pUC18 M13mp18 pBR322
    9 17 2 1 1 2
  • Protocols

    Complete Protocol

    Download PDF

    Restriction Enzyme Protocol

    PDF (410 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

    Other

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