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Flexi Rabbit Reticulocyte Lysate System

by Promega
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  • Overview
  • Protocols
  • Specifications
  • Resources
  • Translation Optimization for Mg2+ and K+ Concentrations

    The Flexi® Rabbit Reticulocyte Lysate System allows translation reactions to be optimized for a wide range of parameters, including Mg2+ and K+ concentrations and the choice to add or omit DTT. To help optimize Mg2+ for a specific message, the endogenous Mg2+ concentration of each lysate batch is stated in the product information included with this product. The Flexi® System also offers the choice of three amino acid mixtures and includes a control RNA encoding the firefly luciferase gene.

    The Flexi® Rabbit Reticulocyte Lysate  System, when optimized, can increase the quantity of protein produced as much as fourfold over a standard lysate reaction. The Flexi® System contains three Amino Acid Mixtures, enabling different choices of radioisotopes. Luciferase Control RNA and Luciferase Assay Reagent are included with the system as a functional control (only full-length luciferase is active).

    For more information, see the Protocols & Applications Guide.

    Applications:

    • Optimizing translation of low-expressing messages.
    • Oxidizing environment can be adjusted, which may be important for maintaining functional activity related to disulfide bridges.
    • Luciferase activity can be used for the detection of inhibitory contaminants in RNA preparations.
    • Investigation of translational control.
    • Screening of chemical libraries for effect on translation.

    Reference:

    1. Beckler, G.S. et al. (1995) Methods in Molecular Biology 37, 215–32.
  • Protocols

    Complete Protocol

    Download PDF

    Flexi® Rabbit Reticulocyte Lysate Systems Technical Bulletin

    PDF (1 MB)

    Quick Protocols

    Download PDF

    Flexi Rabbit Reticulocyte Lysate System Quick Protocol FB041

    PDF (91 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    LESS THAN -65C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

    Citations