accumap-low-ph-protein-digestion-mini-kit

AccuMAP Low pH Protein Digestion Mini Kit

by Promega

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Overview
Protocols
Specifications
Resources

Suppress Nonenzymatic PTMs During Protein Sample Prep

Nonenzymatic posttranslational modifications (PTMs) spontaneously occur in biotherapeutic proteins during manufacturing and storage. The major nonenzymatic PTMs are deamidation, disulfide bond scrambling and oxidation. These modifications can affect efficacy and stability of biotherapeutic proteins and are the subject of careful monitoring.

Nonenzymatic PTMs can also be induced during protein sample preparation for peptide mapping and can compromise analysis. Major causes of nonenzymatic PTMs induced during sample preparation include alkaline pH as well as excipients and impurities that have protein oxidizing activity.
Protocols

Complete Protocol

AccuMAP™ Low pH Protein Digestion Kits

PDF (1 MB)
Certificate of Analysis
Lookup Certificate of Analysis
Storage Conditions

LESS THAN -65C

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.
Resources

Articles
- Sample Preparation Method for Accurate Analysis of Nonenzymatic PTMs in Biotherapeutic Proteins with Peptide Mapping
Citations
- A Multidimensional Analytical Comparison of Remicade and the Biosimilar Remsima
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  2017 Anal. Chem.
Posters
- Characterizing Deamidation, Oxidation with Low pH Peptide Mapping
Other
- How to Avoid Artificial NonEnzymatic PTMs During Peptide SamplePrep