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Suppress Nonenzymatic PTMs During Protein Sample Prep
Nonenzymatic posttranslational modifications (PTMs) spontaneously occur in biotherapeutic proteins during manufacturing and storage. The major nonenzymatic PTMs are deamidation, disulfide bond scrambling and oxidation. These modifications can affect efficacy and stability of biotherapeutic proteins and are the subject of careful monitoring.
Nonenzymatic PTMs can also be induced during protein sample preparation for peptide mapping and can compromise analysis. Major causes of nonenzymatic PTMs induced during sample preparation include alkaline pH as well as excipients and impurities that have protein oxidizing activity.
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Protocols
Complete Protocol
AccuMAP™ Low pH Protein Digestion Kits
PDF (1 MB)
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Certificate of Analysis
Lookup Certificate of AnalysisStorage Conditions
LESS THAN -65C
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
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Resources
Articles
Citations
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A Multidimensional Analytical Comparison of Remicade and the Biosimilar Remsima
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2017 Anal. Chem.
Posters
Other
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A Multidimensional Analytical Comparison of Remicade and the Biosimilar Remsima