AccuMAP Low pH Protein Digestion Mini Kit

Description

• Overview
• Protocols
• Specifications
• Resources

• 

  Suppress Nonenzymatic PTMs During Protein Sample Prep

  Nonenzymatic posttranslational modifications (PTMs) spontaneously occur in biotherapeutic proteins during manufacturing and storage. The major nonenzymatic PTMs are deamidation, disulfide bond scrambling and oxidation. These modifications can affect efficacy and stability of biotherapeutic proteins and are the subject of careful monitoring.

  Nonenzymatic PTMs can also be induced during protein sample preparation for peptide mapping and can compromise analysis. Major causes of nonenzymatic PTMs induced during sample preparation include alkaline pH as well as excipients and impurities that have protein oxidizing activity.

• Protocols

  Complete Protocol

  

  AccuMAP™ Low pH Protein Digestion Kits

  PDF (1 MB)

• Certificate of Analysis

  Lookup Certificate of Analysis

  Storage Conditions

  LESS THAN -65C

  Use Restrictions

  For Research Use Only. Not for Use in Diagnostic Procedures.

• Resources

  

  Articles

  • Sample Preparation Method for Accurate Analysis of Nonenzymatic PTMs in Biotherapeutic Proteins with Peptide Mapping
  

  Citations

  • A Multidimensional Analytical Comparison of Remicade and the Biosimilar Remsima
    --
    2017 Anal. Chem.
  

  Posters

  • Characterizing Deamidation, Oxidation with Low pH Peptide Mapping
  

  Other

  • How to Avoid Artificial NonEnzymatic PTMs During Peptide SamplePrep