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GloSensor cAMP HEK293 Cell Line

by Promega
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  • Overview
  • Protocols
  • Specifications
  • Resources
  • The GloSensor™ cAMP Assay presents a novel approach to measuring cAMP levels in live cells. cAMP is a key second messenger involved in signal transduction of GPCRs acting through G?-s and G?-i proteins. The GloSensor™ cAMP Assay uses a genetically modified form of firefly luciferase into which a cAMP-binding protein moiety has been inserted. Upon binding of cAMP, conformational change is induced leading to increased light output. This live-cell assay excels at kinetic and modulation studies of signaling through cAMP.

    To use the GloSensor™ cAMP Assay the receptor of interest and the biosensor are transiently expressed in a cell line of choice. Alternatively, stably transfected cell lines with both the biosensor and the receptor of interest can be made. The protocol is simple: Cells are pre-equilibrated with GloSensor™ cAMP Reagent for approximately 2 hours; then cells are treated with specific agonists/antagonists or compounds, and luminescence is measured after 10–30 minutes. No other reagent additions or manipulations are required. Any common luminometer with injectors is sufficient to read the assay. GloSensor™ cAMP Reagent is required for use with this assay per the GloSensor™ Limited Use Label License.

  • Protocols

    Complete Protocol

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    GloSensor™ cAMP Assay Technical Manual

    PDF (1 MB)

    Quick Protocols

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    GloSensor cAMP Assay FB113

    PDF (101 KB)

  • Certificate of Analysis

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    Storage Conditions

    BELOW -140C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources