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GoTaq 1-Step RT-qPCR System

by Promega
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  • Relative Quantification of RNA Expression in a Single Reaction

    The GoTaq® 1-Step RT-qPCR System enables detection and relative quantification of RNA expression levels using a one-step RT-qPCR method, combining GoScript™ Reverse Transcriptase and GoTaq® qPCR Master Mix in a single-step real-time amplification reaction.

    The GoScript™ RT Mix for 1-Step RT-qPCR (50X) combines optimized quantities amounts of GoScript™ Reverse Transcriptase, RNasin® Plus RNase Inhibitor and additives to enhance single-step reactions.

    • Achieve Superior Performance: Sensitive detection for earlier quantitation of low- and high-copy-number targets over a broad dynamic range.
    • Rely on a Robust System: Resistant to a wide range of PCR inhibitors.
    • Use a Versatile Product: Compatible with both fast and standard cycling methods on most real-time PCR instruments.
    • Realize Enhanced Stability: Exceptional room-temperature setup is easy for automated and high-throughput detection.

    PCR Satisfaction Guarantee

    Promega's PCR Systems, enzymes and reagents are proven in PCR to ensure reliable, high performance results. Your success is important to us. Our products are backed by a worldwide team of Technical Support scientists. Please contact them for applications or technical assistance. If you are not completely satisfied with any Promega PCR product we will send a replacement or refund your account.

    That's Our PCR Guarantee!
    Product must be within expiration date and have been stored and used in accordance with product literature. See Promega Product Insert for specific tests performed.

    Expression levels for the GRAS gene in oil palm seeds were evaluated via 1-step RT-qPCR using the GoTaq® 1-Step RT-qPCR System (Cat. # A6020).
    Expression levels for the GRAS gene in oil palm seeds were evaluated via 1-step RT-qPCR using the GoTaq® 1-Step RT-qPCR System (Cat. # A6020).
  • Protocols

    Complete Protocol

    Download PDF

    GoTaq® 1-Step RT-qPCR System Technical Manual

    PDF (838 KB)

    Quick Protocols

    Download PDF

    GoTaq OneStep RT qPCR System Quick Protocol FB127

    PDF (199 KB)

    Video Protocols

    VPA6020-fallback
  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

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