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GoTaq 2-Step RT-qPCR System

by Promega
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  • Overview
  • Protocols
  • Specifications
  • Resources
  • Reliable, Dye-Based Detection of RNA Expression Levels

    The GoTaq® 2-Step RT-qPCR System facilitates detection and relative quantification of RNA expression levels via a two-step RT-qPCR method using integrated components: GoScript™ Reverse Transcription System and GoTaq® qPCR Master Mix.

    The master mix is provided as a ready-to-use, stabilized 2X formulation and contains GoTaq® Hot Start Polymerase, MgCl2, dNTPs, BRYT Green® Dye and a proprietary reaction buffer. This master mix contains a low level of carboxy-X-rhodamine (CXR); a separate tube of CXR reference dye is included.

    The GoScript™ Reverse Transcription System includes an optimized reaction buffer and M-MLV reverse transcriptase designed to enable efficient synthesis of first-strand cDNA in preparation for PCR amplification. The cDNA product can be added directly to downstream qPCR amplification reactions.

    Advantages of the GoTaq® 2-Step RT-qPCR System:

    • Sensitive detection for earlier quantitation of low- and high-copy-number targets over a broad dynamic range.
    • Compatible with both fast and standard cycling methods on most real-time PCR instruments.
    • Exceptional room-temperature setup is easy for automated and high-throughput detection.

    Cat.# A6010 contains sufficient reagents for 50 × 20µl RT reactions and 200 × 50µl qPCR reactions; 50 × 20µl RT reactions and 500 × 20µl qPCR reactions; or 50 × 20µl RT reactions and 1,000 × 10µl qPCR reactions.

    PCR Satisfaction Guarantee
    Promega's PCR Systems, enzymes and reagents are proven in PCR to ensure reliable, high performance results. Your success is important to us. Our products are backed by a worldwide team of Technical Support scientists. Please contact them for applications or technical assistance. If you are not completely satisfied with any Promega PCR product we will send a replacement or refund your account.

    That's Our PCR Guarantee!

    Product must be within expiration date and have been stored and used in accordance with product literature. See Promega Product Insert for specific tests performed.

  • Protocols

    Complete Protocol

    Download PDF

    GoTaq® 2-Step RT-qPCR System Technical Manual

    PDF (877 KB)

    Quick Protocols

    Download PDF

    GoTaq® 2-Step RT-qPCR System Quick Protocol FB115

    PDF (73 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources