GoTaq 2-Step RT-qPCR System
Reliable, Dye-Based Detection of RNA Expression Levels
The GoTaq® 2-Step RT-qPCR System facilitates detection and relative quantification of RNA expression levels via a two-step RT-qPCR method using integrated components: GoScript™ Reverse Transcription System and GoTaq® qPCR Master Mix.
The master mix is provided as a ready-to-use, stabilized 2X formulation and contains GoTaq® Hot Start Polymerase, MgCl2, dNTPs, BRYT Green® Dye and a proprietary reaction buffer. This master mix contains a low level of carboxy-X-rhodamine (CXR); a separate tube of CXR reference dye is included.
The GoScript™ Reverse Transcription System includes an optimized reaction buffer and M-MLV reverse transcriptase designed to enable efficient synthesis of first-strand cDNA in preparation for PCR amplification. The cDNA product can be added directly to downstream qPCR amplification reactions.
Advantages of the GoTaq® 2-Step RT-qPCR System:
- Sensitive detection for earlier quantitation of low- and high-copy-number targets over a broad dynamic range.
- Compatible with both fast and standard cycling methods on most real-time PCR instruments.
- Exceptional room-temperature setup is easy for automated and high-throughput detection.
Cat.# A6010 contains sufficient reagents for 50 × 20µl RT reactions and 200 × 50µl qPCR reactions; 50 × 20µl RT reactions and 500 × 20µl qPCR reactions; or 50 × 20µl RT reactions and 1,000 × 10µl qPCR reactions.
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Promega's PCR Systems, enzymes and reagents are proven in PCR to ensure reliable, high performance results. Your success is important to us. Our products are backed by a worldwide team of Technical Support scientists. Please contact them for applications or technical assistance. If you are not completely satisfied with any Promega PCR product we will send a replacement or refund your account.
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Product must be within expiration date and have been stored and used in accordance with product literature. See Promega Product Insert for specific tests performed.
GoTaq® 2-Step RT-qPCR System Technical Manual
PDF (877 KB)
GoTaq® 2-Step RT-qPCR System Quick Protocol FB115
PDF (73 KB)
Certificate of AnalysisLookup Certificate of Analysis
-30C TO -10C
For Research Use Only. Not for Use in Diagnostic Procedures.