Skip to content

GoTaq G2 DNA Polymerase

by Promega
Login to view price.
Size
  • Overview
  • Protocols
  • Specifications
  • Resources
  • GoTaq® G2: Better Performance for PCR

    GoTaq® G2 DNA Polymerase, the second generation of GoTaq® products reliably amplifies a wide range of PCR templates and provides high-performing results due to improved manufacturing processes, increased reliability and consistencyGoTaq® G2 is supplied in a proprietary formulation containing 50% glycerol with buffers designed for enhanced amplification.

    The enzyme is a full-length form of Taq DNA polymerase that exhibits 5´?3´ exonuclease activity. GoTaq® G2 DNA Polymerase is supplied with 5X Green GoTaq® Reaction Buffer and 5X Colorless GoTaq® Reaction Buffer. Both buffers contain MgCl2 at a concentration of 7.5mM for a final concentration of 1.5mM in the 1X reaction. The 5X Green GoTaq® Reaction Buffer has two dyes (blue and yellow) that separate during electrophoresis to indicate migration progress. The buffer also includes a compound that increases sample density so that samples can be loaded directly onto gels without the need for loading dye. The colorless buffer is used when direct fluorescence or absorbance readings are required without prior purification of the amplified DNA from the PCR.

    pcr-guaranteegray-circlePerformance Guarantee: Promega PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any Promega PCR product, we will send a replacement or refund your account.

    GoTaq® G2 DNA Polymerase consistently produces high yields across multiple targets while competitors produce highly variable results.
    GoTaq® G2 DNA Polymerase consistently produces high yields across multiple targets while competitors produce highly variable results.
  • Protocols

    Complete Protocol

    Download PDF

    GoTaq® G2 DNA Polymerase Product Information

    PDF (191 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.