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GoTaq G2 Hot Start Green Master Mix

by Promega
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  • Overview
  • Protocols
  • Specifications
  • Resources
  • Convenient, Room-Temperature Setup and Higher Performance GoTaq® G2 Enzyme

    GoTaq® G2 Hot Start Polymerase is bound to a proprietary antibody that blocks activity at temperatures below 70°C for convenient, room-temperature reaction setup. Polymerase activity is restored during the initial denaturation step, when amplification reactions are heated at 94–95°C for two minutes, allowing hot-start PCR following room-temperature reaction assembly. Hot-start PCR is advantageous for some amplification targets because it may eliminate or minimize primer-dimer and nonspecific products. In some cases, hot-start PCR may improve yields. GoTaq® G2 Hot Start Polymerase also exhibits 5´?3´ exonuclease activity.

    The GoTaq® G2 Hot Start Master Mixes are ready-to-use mixes containing all necessary components (GoTaq® G2 Hot Start Polymerase, buffer, dNTPs and optimized magnesium)—you only need to add primer and template, and go!

    The GoTaq® G2 Hot Start Green Master Mix also contains a gel loading dye to facilitate downstream gel analysis. The GoTaq® G2 Hot Start Colorless Master Mix contains no gel loading dye for use when downstream applications require fluorescence or absorbance readings without purification.

    pcr-guaranteegray-circlePerformance Guarantee: Promega PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any Promega PCR product, we will send a replacement or refund your account.

    GoTaq® G2 Hot Start Master Mix displays superior sensitivity and robust yield.
    GoTaq® G2 Hot Start Master Mix displays superior sensitivity and robust yield.
  • Protocols

    Complete Protocol

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    GoTaq® G2 Hot Start Green Master Mix Protocol

    PDF (214 KB)

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    GoTaq® G2 Hot Start Colorless Master Mix Product Information

    PDF (213 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.