GoTaq Green Master Mix
Set Up PCR in Less than a Minute with Convenient Master Mixes
GoTaq® DNA Polymerase is a proprietary formulation of Taq DNA polymerase that gives robust amplification equal to and, in some cases, superior to that of standard Taq DNA polymerase.
For ultimate in convenience, choose GoTaq® Colorless Master Mix or GoTaq® Green Master Mix and directly substitute in your current PCR application—no need to change cycling parameters.
Both Master Mixes are ready-to-use 2X solutions that contain GoTaq® DNA Polymerase, dNTPs, MgCl2 and reaction buffer at optimal concentrations for efficient amplification of DNA templates by PCR. GoTaq® Green Master Mix also includes two dyes (blue and yellow) that allow monitoring of progress during electrophoresis. The blue dye comigrates at the same rate as 3–5kb DNA fragments in a 1% agarose gel. The yellow dye migrates ahead of primers (<50bp).
GoTaq® Colorless Master Mix has the same formulation as the GoTaq® Green Master Mix but does not include the dyes. Both also include Nuclease-Free Water. Reactions assembled with the GoTaq® Master Mixes have sufficient density for direct loading onto agarose gels.
Reactions can be set up in less than a minute; simply add your template, water and primers and go!
GoTaq® Green and Colorless Master Mix reaction size is based on a 50µl reaction volume.
GoTaq® Green Master Mix (M712) Protocol
PDF (204 KB)
GoTaq® Colorless Master Mix (M713) Product Information
PDF (203 KB)
Certificate of AnalysisLookup Certificate of Analysis
-30C TO -10C
For Research Use Only. Not for Use in Diagnostic Procedures.