GoTaq MDx Hot Start Polymerase, Glycerol-Free
Description
- Overview
- Protocols
- Specifications
- Resources
Consistent and Robust Amplification
GoTaq® MDx Hot Start Polymerase contains GoTaq® MDx DNA Polymerase bound to a proprietary antibody that blocks polymerase activity. The polymerase activity is restored during the initial denaturation step when amplification reactions are heated at 94–95°C for two minutes. This allows hot-start PCR in which polymerase activity is inhibited at temperatures below 70°C, allowing convenient, room-temperature reaction setup. Hot-start PCR is advantageous for some amplification targets because primer-dimer and secondary products are eliminated or minimized. In some cases, hot-start PCR may improve yields. The glycerol-free product formulation is further purified to remove glycerol, making the enzyme suitable for further manufacture processing and lyophilization.
- Manufactured under cGMP using validated equipment, processes and method under a certified Quality System to ensure consistent product performance lot-to-lot.
- QC-tested for bacterial, fungal and mammalian DNA to ensure low DNA contamination.
- Flexible product formats and custom formulations available.
GoTaq® MDx Hot Start Polymerase is a general purpose reagent intended for general laboratory use. It is suitable for use as a component of molecular diagnostic assays, where applicable country laws allow. Separate license or royalty payments not required. This product alone does not provide any diagnostic result.
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Protocols
Complete Protocol
GoTaq® MDx Hot Start Polymerase Product Information
PDF (205 KB)
GoTaq® MDx Hot Start Polymerase, High Concentration, Product Information
PDF (126 KB)
GoTaq® MDx Hot Start Polymerase, Glycerol-Free, Product Information
PDF (204 KB)
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Certificate of Analysis
Lookup Certificate of AnalysisStorage Conditions
-30C TO -10C
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
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Resources
Posters
- Considerations for Use of Taq Polymerase in Molecular Diagnostic Assays Poster
- Evaluation of Manufacture and QC for PCR Components Used in MDx Assays
Other
