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GoTaq Probe qPCR Master Mix

by Promega
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  • 2X Master Mix with GoTaq® Hot Start Polymerase for qPCR

    The GoTaq® Probe qPCR Master Mix is optimized for fast and reproducible quantitative PCR assays in the hydrolysis probe detection format. The master mix is provided as a ready-to-use, stabilized 2X formulation and contains GoTaq® Hot Start Polymerase, MgCl2, dNTPs and a proprietary reaction buffer. This master mix does not contain a reference dye; a separate tube of CXR reference dye is included.

     

    pcr-guaranteegray-circlePerformance Guarantee: Promega PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any Promega PCR product, we will send a replacement or refund your account.

    DNA (10ng) from a lung tumor FFPE sample was amplified using the GoTaq® Probe qPCR Master Mix (Cat.# A6102) under both standard and Fast thermalcycling conditions on a Roche LightCycler 480 instrument using a 384 well plate. Equivalent Cq values were observed for the three genes evaluated regardless of cycling conditions used for the experiment.
    DNA (10ng) from a lung tumor FFPE sample was amplified using the GoTaq® Probe qPCR Master Mix (Cat.# A6102) under both standard and Fast thermalcycling conditions on a Roche LightCycler 480 instrument using a 384 well plate. Equivalent Cq values were observed for the three genes evaluated regardless of cycling conditions used for the experiment.
  • Protocols

    Complete Protocol

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    GoTaq® Probe qPCR Master Mix Technical Manual

    PDF (416 KB)

    Quick Protocols

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    GoTaq Probe qPCR Master Mix Quick Protocol FB138

    PDF (267 KB)

  • Certificate of Analysis

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    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

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