Bioluminescent Detection of GTPase, GAP and GEF Activities
The GTPase-Glo™ Assay is a homogeneous, bioluminescent assay that is used to measure the activities of GTPases and their associated proteins (GEFs and GAPs) in vitro. The assay quantitates the amount of GTP remaining after a GTPase reaction.
With the GTPase-Glo™ Assay, you can measure intrinsic GTPase activity, GAP-stimulated GTPase activity, GAP activity and GEF activity. The kit contains optimized reaction buffers, GTPase/GAP Buffer and GEF buffer, for performing GTPase and GAP reactions, and GEF reactions, respectively.