HaloCHIP System

Covalent Protein:DNA Complex Capture

The HaloCHIP™ System is a novel method designed for the covalent capture of intracellular protein:DNA complexes without the use of antibodies.  HaloCHIP™ System offers an efficient and robust alternative to the standard chromatin immunoprecipitation (ChIP) method. Proteins of interest are expressed in cells as HaloTag® fusion proteins, crosslinked to DNA with formaldehyde and then captured on HaloLink™ Resin, which forms a highly specific, covalent interaction with the HaloTag® portion of the fusion protein. Stringent washing removes nonspecific proteins and DNA, and heating reverses the crosslinks between the DNA and the fusion protein and releases the captured DNA fragment, which subsequently can be purified.

The HaloCHIP™ System helps you obtain data in 24–48 hours with fewer steps, minimizing potential experimental errors, and with improved signal-to-noise ratios, to detect small changes in protein-binding from a minimal number of cells.

Application

• Protein:DNA interaction analysis.