HaloTag Mammalian Pull-Down System
Streamline Your Protein Purification Process
The HaloTag® Mammalian Pull-Down Systems are designed to capture and purify intracellular binary and higher order protein complexes, including transient or weakly interacting partners.
HaloTag® Mammalian Pull-Down System (Cat.# G6504) includes buffers and resin necessary to perform a HaloTag® pull-down.
HaloTag® Mammalian Pull-Down and Labeling System (Cat.# G6500) includes everything in G6504 plus the HaloTag® TMRDirect™ Ligand, which allows correlative cellular localization and real-time imaging studies.
HaloTag® Complete Pull-Down System (Cat.# G6509) includes everything in the Mammalian Pull-Down and Labeling System (Cat.# G6500) plus a starter cloning system, Wizard® SV Gel and PCR Clean-Up System, and FuGENE® HD Transfection Reagent.
HaloTag® Control Vector provides protein expression of the HaloTag® protein in mammalian cells, E. coli or in vitro expression systems dependent on human cytomegalovirus (CMV) intermediate early enhancer, T7 or SP6 RNA polymerase promoters. It can be used as a control for any HaloTag® experimental system and can be used for both stable and transient HaloTag® expression in mammalian cells; for stable expression, co-transfection with a vector containing a selectable marker is required.
These pull-down methods are compatible with mass spectrometry analysis.
GenBank®/EMBL Accession Number and Sequence File
HaloTag® Complete Pull-Down System Technical Manual
PDF (24 MB)
HaloTag® Mammalian Pull-Down and Labeling Systems Technical Manual
PDF (676 KB)
Certificate of AnalysisLookup Certificate of Analysis
COMPONENTS AT DIFFERENT TEMPS
For Research Use Only. Not for Use in Diagnostic Procedures.