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HB101 Competent Cells, >10(8)cfu/ug

by Promega
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  • Overview
  • Protocols
  • Specifications
  • Resources
  • HB101 cells are recA–, which minimizes recombination events, and contain a restriction-minus background (hsdS20) that allows better representation when cloning methylated DNA. HB101 Competent Cells are suitable for vectors that do not require ?-complementation for blue/white screening.

    For researchers doing more than one transformation, competent cells are available in standard format (200µl aliquots). For added convenience, single-use competent cells (50µl aliquots) also are offered.

    HB101 Competent Cells are prepared according to a modified procedure of Hanahan.

    HB101 Genotype: F–, thi-1, hsdS20 (rB, mB), supE44, recA13, ara-14, leuB6, proA2, lacY1, galK2, rpsL20 (strr), xyl-5, mtl-1.

    Reference

    1. Hanahan, D. (1985) DNA Cloning, Vol. 1, Glover, D., ed., IRL Press, Ltd., 109.
  • Protocols

    Complete Protocol

    Download PDF

    E. coli Competent Cells Technical Bulletin

    PDF (686 KB)

    Quick Protocols

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    E Coli Competent Cells Quick Protocol FB035

    PDF (175 KB)

  • Certificate of Analysis

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    Storage Conditions

    LESS THAN -65C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.