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HisLink Spin Protein Purification System

by Promega
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  • Protein Purification from Bacterial Cultures; You Choose the Method

    HisLink™ Protein Purification Systems are designed for purification of polyhistidine (His)-tagged or HQ-tagged proteins directly from culture medium containing bacterial cells expressing the tagged protein of interest. Both the "spin" and "96" formats use the HisLink™ Protein Purification Resin (Cat.# V8821), a macroporous silica resin derivatized with a high level of tetradentate-chelated nickel (>20mmol Ni/ml settled resin).

    The resin performs well in either column, batch or vacuum-based methods with a binding capacity of >15mg/ml of resin. HisLink™ Protein Purification Resin is useful in all general immobilized metal affinity chromatography (IMAC) applications matrix as well as in low- to medium-pressure liquid chromatography systems.

    The bacterial cells are lysed using the FastBreak™ Cell Lysis Reagent (Cat.# V8573), and the crude lysate is combined with the HisLink™ Resin. The addition of these reagents results in simultaneous bacterial lysis and binding of the polyhistidine- or HQ-tagged proteins. No long lysozyme incubations are required for cell lysis.

    The resin can be used with vacuum filtration devices (e.g., Vac-Man® Vacuum Manifold [Cat.# A7231]) to rapidly process simultaneous samples. The HisLink™ 96 Protein Purification System (Cat.# V3680) provides a simple and quick vacuum-based method of simultaneously purifying multiple His-tagged proteins  from E. coli cultured in deep-well, 96-well plates. The HisLink™ 96 System is amenable to manual or automated methods, such as the Beckman Coulter Biomek® 2000 or FX for high-throughput applications, yielding 1mg of purified polyhistidine-tagged protein per well.

    Note: Resin product volumes represent total volume of a 50% resin slurry.

    Applications

    • Protein purification of polyhistidine- or HQ-tagged proteins using IMAC.
    • Purification of tagged proteins from E. coli using a vacuum- or centrifugation-based format.
    • Protein purification of polyhistidine- or HQ-tagged proteins from 96-well, deep-well culture plates using IMAC.

    For more information, see the Protocols & Applications Guide.

    Overview of polyhistidine- or HQ-tagged protein purification using the HisLink™ Spin Protein Purification System.
    Overview of polyhistidine- or HQ-tagged protein purification using the HisLink™ Spin Protein Purification System.
  • Protocols

    Complete Protocol

    Download PDF

    HisLink™ 96 Protein Purification System Technical Bulletin

    PDF (878 KB)

    Download PDF

    HisLink™ Spin Protein Purification System Technical Manual

    PDF (543 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    +2C TO +10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

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