- Overview
- Protocols
- Specifications
- Resources
IgG-Digesting Enzymes for Therapeutic Antibody Characterization
IdeS and IdeZ Protease are immunoglobulin G (IgG)-degrading enzymes that are valuable tools for the characterization of therapeutic antibodies, Fc fusion proteins and antibody-drug conjugates.
IdeS and IdeZ Proteases are fast, easy-to-use proteases; complete digestion can be achieved in 30 minutes without optimization. Cleavage is highly reproducible and specific, at a single site below the hinge region.
Both IdeS and IdeZ Proteases effectively cleave human IgG1, IgG2, IgG3 and IgG4, monkey, sheep, rabbit, humanized and chimeric IgGs as well as Fc-fusion proteins. However, only IdeZ Protease cleaves mouse IgG2a and IgG3.
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Protocols
Complete Protocol
IdeS Protease Protocol
PDF (256 KB)
Ides Protease, Frozen, Protocol
PDF (254 KB)
IdeZ Protease, Lyophilized, Protocol
PDF (361 KB)
IdeZ Protease, Frozen, Protocol
PDF (361 KB)
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Certificate of Analysis
Lookup Certificate of AnalysisStorage Conditions
-30C TO -10C
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
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Resources
Citations
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Evaluation of automated Wes system as an anlytical and characterization tool to support monoclonal antibody drug product development
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2017 J. Pharm. Biomed. Anal.
Posters
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Evaluation of automated Wes system as an anlytical and characterization tool to support monoclonal antibody drug product development
