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Immobilized Trypsin

by Promega
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  • Overview
  • Protocols
  • Specifications
  • Resources
  • Immobilized Trypsin provides a fast and convenient method for digesting a range of concentrations of purified protein or complex protein mixtures. Digested peptides are easily separated from the Immobilized Trypsin as they flow through the spin column into the collection tube. Immobilized Trypsin is easily removed from the peptide solution because the trypsin does not pass though the column frit. Trypsin is a proteolytic enzyme, which cleaves at the carboxyl side of positively charged Lysine (Lys) and Arginine (Arg). When these amino acids are followed by the nonpolar Proline (Pro), the digestion of the site is not efficient. When Lys and Arg are followed by acids [Aspartic Acid (Asp) and Glutamic Acid (Glu)] the digestion is also not as efficient.

    Immobilized Trypsin lets you perform digestions in as little as 30 minutes. The easily adjustable protocol easily accommodates various protein concentrations. No shaking or water baths needed.

    Applications

    • Protein identification
    • Quantitative analysis
    • Differential expression
    • Post-translational modifications
    Overview of the Immobilized Trypsin digestion protocol.
    Overview of the Immobilized Trypsin digestion protocol.
  • Protocols

    Complete Protocol

    Download PDF

    Immobilized Trypsin Technical Manual

    PDF (195 KB)

    Quick Protocols

    Download PDF

    Immoblilized Trypsin Quick Protocol FB105

    PDF (44 KB)

    Video Protocols

    VPV9012-fallback
  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    +2C TO +10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

    Articles