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ImProm-II Reverse Transcription System

by Promega
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  • Overview
  • Protocols
  • Specifications
  • Resources
  • Complete System for First-Strand cDNA Synthesis of Long or Rare Messages

    The ImProm-II™ Reverse Transcription System produces efficient, robust synthesis of first-strand cDNA in preparation for PCR amplification. The components of the ImProm-II™ Reverse Transcription System can be used to reverse transcribe RNA templates starting with total RNA, poly(A)+ mRNA or synthetic transcript RNA. The optimized reaction buffer and powerful ImProm-II™ Reverse Transcriptase provided in the ImProm-II™ System together enable robust, full-length cDNA synthesis for the reproducible analysis of rare or long messages. The cDNA synthesis conditions were formulated for standalone applications or for easy transition to gene-specific target amplification. An aliquot of the reverse transcription reaction (1–20ul) can be amplified directly using Taq DNA polymerase in coupled or uncoupled PCR.

    • Amenable to Full-Length RT-PCR: Reverse transcribe long RNA templates up to 8.9kb.
    • Microarray-Compatible: May be used to incorporate regular, Cy®3-modified, Cy®5-modified and amino-allyl-modified nucleotides.
    • Easy to Use: System provides all reagents necessary for efficient reverse transcription.
    • Scalable and Flexible: 1–20ul of the initial reverse transcription reaction may be used in subsequent PCR, and the optimized buffer allows coupled RT-PCR.
    • RT Provided with 5X Reaction Buffer: 250mM Tris-HCl (pH 8.3 at 25°C), 375mM KCl and 50mM DTT. A 25mM MgCl2 Solution also is included.
    • Versatile: Use with your thermostable DNA polymerase of choice.
    • Choose Your Configuration: Learn more about our custom options for this product at:
  • Protocols

    Complete Protocol

    Download PDF

    ImProm-II™ Reverse Transcription System Technical Manual

    PDF (1 MB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources