Note: I-PpoI recognizes rDNA repeats (approximately 200) in S. cerevisiae chromosome 12, generating 600bp, 400bp and 9kb fragments.
Incubation Conditions: I-PpoI Buffer. 37°C.
Source: I-PpoI is an intron-encoded endonuclease from Physarum polycephalum. The enzyme is produced from a recombinant E. coli strain.
Storage Buffer: 50mM Tris-HCl (pH 7.5), 0.01% NP-40, 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.
Percent Activity in 4-CORE® Buffer:
A B C D I-PPoI 10–25% 25–50% 25–50% 25–50% 100%
Note: A precipitate may be observed after thawing. If detected, heat to 37°C for 3–5 minutes and mix to dissolve. The buffer may appear cloudy after heating; this will not affect the performance of the product.
Frequency of Cutting: No sites are present in any of the commonly used plasmids or vectors. One site is present in chromosome 12 of S. cerevisiae DNA.
Restriction Enzyme Protocol
PDF (410 KB)
Certificate of AnalysisLookup Certificate of Analysis
-30C TO -10C
For Research Use Only. Not for Use in Diagnostic Procedures.