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I-PpoI

by Promega
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  • Overview
  • Protocols
  • Specifications
  • Resources
  • Cut Site:

    CTCTC    TTAA?GGTAGC
    GAGAG?AATT   CCATCG

    Note: I-PpoI recognizes rDNA repeats (approximately 200) in S. cerevisiae chromosome 12, generating 600bp, 400bp and 9kb fragments.

    Incubation Conditions: I-PpoI Buffer. 37°C.

    Source: I-PpoI is an intron-encoded endonuclease from Physarum polycephalum. The enzyme is produced from a recombinant E. coli strain.

    Storage Buffer: 50mM Tris-HCl (pH 7.5), 0.01% NP-40, 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

    Percent Activity in 4-CORE® Buffer:

    A B C D I-PPoI
    10–25% 25–50% 25–50% 25–50% 100%

    Note: A precipitate may be observed after thawing. If detected, heat to 37°C for 3–5 minutes and mix to dissolve. The buffer may appear cloudy after heating; this will not affect the performance of the product.

    Frequency of Cutting: No sites are present in any of the commonly used plasmids or vectors. One site is present in chromosome 12 of S. cerevisiae DNA.

  • Protocols

    Complete Protocol

    Download PDF

    Restriction Enzyme Protocol

    PDF (410 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

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