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ISOQUANT Isoaspartate Detection Kit

by Promega
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  • Overview
  • Protocols
  • Specifications
  • Resources
  • Monitor Protein Deamidation through the Sensitive Detection of Isoaspartate

    The ISOQUANT® Isoaspartate Detection Kit is intended for quantitative detection of isoaspartic acid residues in proteins and peptides, which can result from the gradual, nonenzymatic deamidation of asparagine or rearrangement of aspartic acid residues during storage or handling. Because the kit does not depend on the monitoring of charge differences for detection, charge heterogeneity does not interfere with the assay. The ISOQUANT® Kit can be used on peptides or proteins such as monoclonal antibodies.

    Advantages

    Great Efficiency: Simple procedure with a test time of less than one hour. Automation possible with HPLC autosampler capability.

    Economical: HPLC detection eliminates cost and inconvenience of radioactive materials handling.

    Analytical: Quantitative results available.

    Versatile: Perform individual samples or batches. Small sample size makes the assay suitable for research, analytical methods, formulations and process development work.

    Robust: Not affected by common buffer components.

    Flexible: Fits with existing equipment and expertise.

    Sensitive: Detects isoaspartate resulting from aspartic acid rearrangement as well as deamidation of asparagine.

  • Protocols

    Complete Protocol

    Download PDF

    ISOQUANT® Isoaspartate Detection Kit Technical Bulletin

    PDF (411 KB)

    Quick Protocols

    Download PDF

    ISOQUANT Isoaspartate Detection Kit Quick Protocol FB078

    PDF (88 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

    Citations