Kinase-Glo Luminescent Kinase Assay
Luminescent, High-Throughput Kinase Activity Assays
Simple, Scalable and Automation-Friendly
The Kinase-Glo® Assays are designed for use with multiwell plate formats, making them ideal for automated high-throughput screening (HTS), and they can be used to assay protein, lipid and sugar kinases. The assay procedure involves addition of a single reagent directly to a completed kinase reaction. This addition results in the generation of a luminescent signal correlated with the amount of ATP present and inversely proportional to the amount of kinase activity.
The assay produces excellent Z´-factor values of greater than 0.7 in 96- and 384-well formats, easily detects known kinase inhibitors and provides IC50 values comparable to those reported in the literature. Validated automated methods available at: www.promega.com/automethods/
Choose Your Assay FormatThe three Kinase-Glo® Assay systems are differentiated by their linear response to ATP. The original Kinase-Glo® Assay is linear to 10uM ATP, while Kinase-Glo® Plus Assay is linear to 100uM ATP. The newest assay, Kinase-Glo® Max, is linear to 500uM ATP, making it well suited for use with kinases with high Km for ATP as well as for screening for kinase inhibitors that do not compete at the ATP binding site.
Kinase-Glo® Luminescent Kinase Assay Platform Technical Bulletin
PDF (1 MB)
Certificate of AnalysisLookup Certificate of Analysis
-30C TO -10C
For Research Use Only. Not for Use in Diagnostic Procedures.