ADP-Glo Kinase Assay + AMPK (A1/B1/G2) Kinase Enzyme System
Convenient, Scalable Kinase Profiling
The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The AMPK (A1/B1/G2) Kinase Enzyme System contains:
- AMPK (A1/B1/G2), 10ug (Human, recombinant full-length). MW: ~68kDa (A1); ~38kDa (B1); ~65kDa (G2).
- SAMStide Substrate (HMRSAMSGLHLVKRR); derived from the mouse acetyl-Coenzyme A carboxylase alpha (amino acids 73–85).
- Reaction Buffer, DTT, AMP Solution.
Recombinant full-length human AMPK (combination of A1/B1/G2 subunits) was expressed by baculovirus in Sf9 insect cells using a C-terminal His tag. AMP-activated protein kinase (AMPK) exhibits a key role as a master regulator of cellular energy homeostasis.
The AMPK (A1/B1/G2) Kinase Enzyme System can be purchased with or without the ADP-Glo™ Kinase Assay reagents. Used together, the ADP-Glo™ Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.
Use with ADP-Glo™ Kinase Assay
The ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.
See all Kinase Enzyme Systems available from Promega.
AMPK (A1/B1/G2) Kinase Enzyme Protocol
PDF (943 KB)
Certificate of AnalysisLookup Certificate of Analysis
LESS THAN -65C
For Research Use Only. Not for Use in Diagnostic Procedures.