- Overview
- Protocols
- Specifications
- Resources
The pro-luciferin substrates can be used to monitor the activity of specific isoforms of cytochrome P450 or NAT2 as indicated in the name of the substrate. The Luciferin-MultiCYP is a promiscuous substrate that reacts with at least 21 P450 isoforms and is useful for measuring net CYP activity in a mixed population of P450s. The Luciferin-NAT2 is an excellent substrate for N-acetyltransferase 2 (NAT2), a phase II biotransformation enzyme that acetylates aromatic amine groups on xenobiotic compounds. This substrate shows little to no cross-reactivity with NAT1.
Luciferin Detection Reagent must be used in combination with the pro-luciferin substrates to monitor the activity of specific isoforms of cytochrome P450 or NAT 2 as indicated in the name of the substrate.
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Certificate of Analysis
Lookup Certificate of AnalysisStorage Conditions
-30C TO -10C
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
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Resources
Articles
- Bioluminescent N-Acetyl Transferase-2 Enzyme Assay Using a Novel Luciferin Derivative as a Substrate
- Nonselective Cytochrome P450 Enzyme Assay Using a Novel Bioluminescent Probe Substrate that Cross-reacts with Multiple P450 Enzymes
- Cytochrome P450 2J2 Enzyme Assay Using a Novel Bioluminescent Probe Substrate
Citations
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Assembly of Hepatocyte Spheroids Using Magnetic 3D Cell Culture for CYP450 Inhibition/Induction
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2017
Other
- Cytochrome P450 4F2 and 4F3B Enzyme Assays Using a Novel Bioluminescent Probe Substrate
- Cytochrome P450 4F12 Enzyme Assay Using a Novel Bioluminescent Probe Substrate
- Selective Cytochrome P450 3A7 Enzyme Assay Using a Novel Bioluminescent Probe Substrate
- Cytochrome P450 2J2 Enzyme Assay Using a Novel Bioluminescent Probe Substrate
- Nonselective Cytochrome P450 Enzyme Assay Using a Novel Bioluminescent Probe Substrate that Cross-reacts with Multiple P450 Enzymes
- P450-Glo™ Assays Protocol
