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MagneHis Protein Purification System

by Promega
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  • Overview
  • Protocols
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  • Purify Polyhistidine (His-) or HQ-Tagged Proteins

    MagneHis™ Protein Purification System provides a simple, rapid, reliable method for the purification of polyhistidine- or HQ-tagged, expressed proteins. Paramagnetic precharged nickel particles (MagneHis™ Ni-Particles) are used to isolate polyhistidine- or HQ-tagged protein directly from a crude cell lysate using either a manual (requires use of a magnetic stand) or automated procedure. Using a tube format, polyhistidine- or HQ-tagged protein can be purified on a small scale using less than 1ml of culture or on a large scale using more than 1 liter of culture. Samples can be processed in a high-throughput manner using a robotic platform such as the Beckman Coulter Biomek® 2000 or FX or Tecan Genesis® RSP.

    MagneHis™ Ni-Particles are compatible with a variety of common buffers and are suitable for use with cultures volumes from 1ml–1liter. Binding capacity is approximately 1mg of polyhistidine-tagged protein per 1ml of MagneHis™ Ni-Particles.

    Note: This product requires the use of a magnetic stand.

    For more information, see the Protocols & Applications Guide.

    Applications
    • Protein purification of polyhistidine- or HQ-tagged, expressed proteins from a crude E. coli cell lysate.
    • High-throughput purification of His- or HQ-tagged proteins using automated platforms.
    • Purification of His-tagged proteins from insect or mammalian cell lysates.
    • Pull-down of His-tagged proteins for protein:protein interaction studies.

     

     

    Purification of His-tagged methionyl tRNA synthetase.
    Purification of His-tagged methionyl tRNA synthetase.
  • Protocols

    Complete Protocol

    Download PDF

    MagneHis™ Protein Purification System Technical Manual

    PDF (1 MB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    +2C TO +10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

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