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MagneSil Total RNA mini-Isolation System

by Promega
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  • Overview
  • Protocols
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  • The MagneSil® Total RNA mini-Isolation System provides a high-throughput 96-well format for fast, simple preparation of intact total RNA from small amounts of cell culture (?1 × 105 tissue culture cells), tissue (?2mg tissue lysate in 100ul) or freshly isolated whole blood (?20ul). The protocol enables high-throughput automated purification on a variety of liquid-handling workstations. Isolation of total RNA in a 384-well format from cell culture (?1 × 103 cells) and freshly isolated whole blood (?5ul) also may be performed. Total RNA purification is achieved without vacuum filtration, centrifugation or precipitation. The 96-well procedure takes about 30 minutes to complete using a liquid-handling workstation.

    Total RNA purified using this system is suitable for a variety of molecular biology applications including endpoint RT-PCR amplification and real-time RT-PCR.

    • Only 30 minutes are required to process one 96-well plate, or 50 minutes for one 384-well plate on a Beckman Coulter Biomek® FX liquid handler.
    • Elution volumes as low as 15ul provide concentrated RNA without the need for time-consuming vacuum concentration.
    • DNase I treatment is included to remove genomic DNA contamination.
    • Validated automated methods are available at: www.promega.com/automethods/
  • Protocols

    Complete Protocol

    Download PDF

    MagneSil® Total RNA mini-Isolation System Technical Bulletin

    PDF (292 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    +15C TO +30C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

    Articles

    Software and Firmware