- Overview
- Protocols
- Specifications
- Resources
Faster, Safer, Hands-Free DNA Purification from FFPE Samples
The Maxwell® 16 FFPE Plus LEV DNA Purification Kit is used to extract DNA rapidly from formalin-fixed, paraffin-embedded tissue samples using the Maxwell® 16 Instrument. The kit is designed for optimal purification of DNA from one to ten 5um thin sections of FFPE tissue samples, maximizing yield and eliminating the need to concentrate the extract prior to amplification.
Time-Saving Protocol Options for Efficient Processing
The protocol provides overnight and 1-hour options for proteinase K digestion of samples prior to DNA purification. After proteinase K digestion, tissue lysates are placed directly into the FFPE Kit cartridges and amplification-ready genomic DNA is obtained in approximately 30 minutes. The Maxwell® 16 Instrument processes 1–16 cartridges per run. The kit does not use hazardous xylene, providing a much safer method than other FFPE purification products. Quality testing demonstrates virtually no PCR inhibitors in purified samples.
Maxwell® Cartridge Seal Change
The seal length on Maxwell® Cartridges has changed to create a pull-tab feature. The manufacturing procedures and quality-control testing of products have not changed.
If you have any questions, please contact us:
In North America, call Promega Technical Services at 800-356-9526.
Outside North America, contact your branch office or call Promega Technical Services at 608-274-4330.
Worldwide, e-mail Promega Technical Services at:techserv@promega.com-
Protocols
Complete Protocol
Maxwell® 16 FFPE Plus LEV DNA Purification Kit Technical Manual
PDF (573 KB)
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Certificate of Analysis
Lookup Certificate of AnalysisStorage Conditions
+15C TO +30C
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
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Resources
Articles
- Quantitating Maxwell FFPE DNA Samples Using the QuantiFluor dsDNA System and the Quantus Fluorometer
Citations
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Comparison of Pre-Analytical FFPE Sample Preparation Methods and Their Impact on Massively Parallel Sequencing in Routine Diagnostics
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2014 PLos ONE
Software and Firmware
Other