ADP-Glo Kinase Assay + CAMK2 alpha Kinase Enzyme System
Convenient, Scalable Kinase Profiling
The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The CAMK2? Kinase Enzyme System contains:
- CAMK2? Kinase, 10ug (Human, recombinant full-length). MW: ~74kDa.
- Autocamtide 2 Peptide Substrate (KKALRRQETVDAL-amide); derived from the autophosphorylation site (amino acids 283–290) on CaMKII.
- Reaction Buffer, DTT, Ca2+/Calmodulin solution.
Recombinant full-length human CAMK2? was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. CAMK2? is a ser/thr protein kinase that is a member of the Ca2+/calmodulin-dependent protein kinase family. CAMK2? is abundant in the brain as a major constituent of the postsynaptic density and is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its Ca2+/calmodulin-dependent activity, CAMK2? can undergo autophosphorylation, resulting in Ca2+/calmodulin-independent activity. The protein level of CAMK2? fluctuates during neuronal activity in cultured rat pup hippocampal neurons. The levels of CAMK2? increased with heightened neuronal activity.
The CAMK2? Kinase Enzyme System can be purchased with or without the ADP-Glo™ Kinase Assay reagents. Used together, the ADP-Glo™ Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.
Use with ADP-Glo™ Kinase Assay
The ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.
See all Kinase Enzyme Systems available from Promega.
CAMK2 alpha Kinase Enzyme Protocol
PDF (1 MB)
Certificate of AnalysisLookup Certificate of Analysis
LESS THAN -65C
For Research Use Only. Not for Use in Diagnostic Procedures.