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Maxwell 16 LEV RNA FFPE Kit

by Promega
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  • Overview
  • Protocols
  • Specifications
  • Resources
  • Safe, Hands-Free RNA Purification from 1–16 FFPE Samples

    The Maxwell® 16 LEV RNA FFPE Purification Kit is designed to extract RNA rapidly from FFPE samples using the Maxwell® 16 Instrument (Cat.# AS2000 and AS3000). The FFPE kit is specifically designed for optimal purification of RNA from 5um thin sections of formalin-fixed, paraffin-embedded (FFPE) tissue samples.

    The Maxwell® 16 LEV RNA FFPE Purification Kit is optimized to yield a final RNA concentration that maximizes RNA yield, eliminating the need to concentrate the extract prior to amplification. Lysates are placed directly into the cartridges, and amplification-ready RNA is obtained in approximately 60 minutes (after proteinase K digest). This kit does not use hazardous xylene, thereby providing a much safer method than many competitive kits. Additionally, quality testing demonstrates virtually no PCR inhibitors in purified samples.

    • Provides high yield of pure amplifiable RNA from FFPE mammalian tissue samples, minimizing sample waste and reruns.
    • Simple method with no organic solvents used for deparaffinization.
    • Protocol can be completed within a single 8-hour shift.

    The purified RNA is suitable for downstream applications including RT-PCR, RT-qPCR, endpoint RT-PCR, cDNA library synthesis and gene expression analysis.

  • Protocols

    Complete Protocol

    Download PDF

    Maxwell® 16 LEV RNA FFPE Kit Technical Manual

    PDF (1 MB)

    Quick Protocols

    Download PDF

    Maxwell 16 LEV RNA FFPE Kit FB167

    PDF (57 KB)

  • Certificate of Analysis

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    Storage Conditions

    +15C TO +30C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.