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MboI

by Promega
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  • Overview
  • Protocols
  • Specifications
  • Resources
  • Cut Site:

    ?GATC
       CTAG?

    Notes:

    1. Sensitive to dam methylation.
    2. Sau3AI is an isoschizomer of MboI that is not sensitive to dam methylation.
    3. DpnI is an unusual isoschizomer of MboI that will cut only if the recognition site is methylated by the dam methylation system.

    Incubation Conditions: Buffer C. 37°C.

    Source: Moraxella bovis ATCC 10900.

    Storage Buffer: 10mM Tris-HCl (pH 7.4), 50mM KCl, 0.1mM EDTA, 0.2mg/ml BSA, 50% glycerol.

    Percent Activity in 4-CORE® Buffer:

    A B C D MULTI-CORE™
    10–25% 75–100% 100% 50–75% <10%

    Frequency of Cutting:

    ? Ad-2 ?X174 pUC18 M13mp18 pBR322
    116 87 0 15 6 22
  • Protocols

    Complete Protocol

    Download PDF

    Restriction Enzyme Protocol

    PDF (410 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

    Other