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mFcgammaRIV ADCC Bioassay Effector Cells, Propagation Model

by Promega
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  • Protocols
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  • Measure Activity of Mouse Antibodies that Bind and Activate mFc?RIV

    Antibody-dependent cell-mediated cytotoxicity (ADCC) is an important mechanism of action (MOA) of antibodies that target virus-infected or diseased (e.g., tumor) cells for destruction by components of the cell-mediated immune system. Mouse Fc?RIV (mFc?RIV) is the predominant receptor involved in ADCC in the mouse and is more closely related to human Fc?RIIIa, the primary Fc receptor involved in ADCC in humans, than mFc?RIII.

    The mFc?RIV ADCC Reporter Bioassay is a biologically relevant MOA-based assay that can be used to measure the activity of mouse antibodies that specifically bind and activate Fc?RIV. Mouse IgG2a, and to a lesser extent IgG2b, are known to mediate ADCC through the activation of mFc?RIV. In contrast, mouse IgG1 does not bind to mFc?IV. The bioassay overcomes the limitations of more labor-intensive and highly variable primary cell assays. The bioassay workflow is simple and robust, compatible with 96-well and 384-well plate formats and, unlike traditional primary cell-based assays, provides a quantitative measure of ADCC with low variability and high accuracy.

    mFc?RIV ADCC Bioassay Effector Cells, Propagation Model, allows propagation and banking of the mFc?RIV Effector Cells. Bio-Glo™ Luciferase Assay System is the required reagent for use with mFc?RIV ADCC Bioassay Effector Cells, Propagation Model.

  • Protocols

    Complete Protocol

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    mFc?RIV ADCC Reporter Bioassay Effector Cells, Propagation Model Technical Manual

    PDF (1019 KB)

  • Certificate of Analysis

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    Storage Conditions

    BELOW -140C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.