- Overview
- Protocols
- Specifications
- Resources
Demonstrate Antibody ADCC Activity Through the mFc?RIV Receptor
Antibody-dependent cell-mediated cytotoxicity (ADCC) is an important mechanism of action (MOA) of antibodies that target virus-infected or diseased (e.g., tumor) cells for destruction by components of the cell-mediated immune system. Mouse Fc?RIV (mFc?RIV) is the predominant receptor involved in ADCC in the mouse and is more closely related to human Fc?RIIIa, the primary Fc receptor involved in ADCC in humans, than mFc?RIII.
The mFc?RIV ADCC Reporter Bioassay is a biologically relevant MOA-based assay that can be used to measure the activity of mouse antibodies that specifically bind and activate Fc?RIV. Mouse IgG2a, and to a lesser extent IgG2b, are known to mediate ADCC through the activation of mFc?RIV. In contrast, mouse IgG1 does not bind to mFc?IV. The bioassay overcomes the limitations of more labor-intensive and highly variable primary cell assays. The bioassay workflow is simple and robust, compatible with 96-well and 384-well plate formats and, unlike traditional primary cell-based assays, provides a quantitative measure of ADCC with low variability and high accuracy.
Applications
Demonstrate antibody ADCC activity.
Confirm mFc?RIV-mediated MOA.
Measure antibody potency and stability.
Screen and rank-order antibodies.
Conveniently Available in a Format that Meets Your Needs
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Complete Kit
Includes mFc?RIV Effector Cells, Target Cells (Raji), Control Antibody, Cell Culture Medium and Assay Reagents.
Recommended for use as a starter kit. -
Core Kits
Include mFc?RIV Effector Cells, Cell Culture Medium and Assay Reagents.
Recommended for routine use with customer-defined antibody and target cells.
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Complete Kit
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Protocols
Complete Protocol
mFc?RIV ADCC Reporter Bioassay, Complete Kit, Technical Manual
PDF (1 MB)
mFc?RIV ADCC Reporter Bioassay, Core Kit, Technical Manual
PDF (947 KB)
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Certificate of Analysis
Lookup Certificate of AnalysisStorage Conditions
COMPONENTS AT DIFFERENT TEMPS
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
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Resources
Posters
- A human FcgRIIa (H131 variant)-specific reporter assay for evaluating antibody activity in ADCP pathway activation poster
- Fc Effector Bioassays for Rapid Quantitative Measurement of ADCC and ADCP Mechanisms of Action Poster
- Fc Effector Reporter Bioassays for Rapid and Quantitative Measurement of ADCC and ADCP Mechanisms of Action
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