M-MLV Reverse Transcriptase
RNA-Dependent DNA Polymerase for cDNA Synthesis
Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long messenger RNA templates (>5kb). The enzyme is a product of the pol gene of M-MLV and consists of a single subunit with a molecular weight of 71kDa. The RNase H activity of M-MLV RT is weaker than that of the commonly used Avian Myeloblastosis Virus (AMV) reverse transcriptase.Advantages
- Provided with 5X Reaction Buffer: 250mM Tris-HCl (pH 8.3 at 25°C), 375mM KCl, 15mM MgCl2, 50mM DTT.
- May Be Heat-Inactivated: M-MLV RT is inactivated by heating at 70°C for 10 minutes.
- Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/
M-MLV Reverse Transcriptase Protocol
PDF (110 KB)
Certificate of AnalysisLookup Certificate of Analysis
-30C TO -10C
For Research Use Only. Not for Use in Diagnostic Procedures.