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M-MLV Reverse Transcriptase

by Promega
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  • Overview
  • Protocols
  • Specifications
  • Resources
  • RNA-Dependent DNA Polymerase for cDNA Synthesis

    Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long messenger RNA templates (>5kb). The enzyme is a product of the pol gene of M-MLV and consists of a single subunit with a molecular weight of 71kDa. The RNase H activity of M-MLV RT is weaker than that of the commonly used Avian Myeloblastosis Virus (AMV) reverse transcriptase.

    Advantages
    • Provided with 5X Reaction Buffer: 250mM Tris-HCl (pH 8.3 at 25°C), 375mM KCl, 15mM MgCl2, 50mM DTT.
    • May Be Heat-Inactivated: M-MLV RT is inactivated by heating at 70°C for 10 minutes.
    • Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/
  • Protocols

    Complete Protocol

    Download PDF

    M-MLV Reverse Transcriptase Protocol

    PDF (110 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

    Citations